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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in many tumors, which ends up in chromosome instability, and so might lead to tumorigenesis. This protein was observed to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence info just isn't nevertheless offered.
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Blocking peptides are peptides that bind precisely for the target antibody and block antibody binding. These peptide ordinarily consists of the epitope identified by the antibody.
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This tutorial outlines even more achievable optimization of mobile and immunoassay parameters to make sure the very best effects are...
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This guidebook outlines further feasible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope about the concentrate on protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin household, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic occasion. Cyclin E sorts a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and is degraded as cells development by means of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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